Journal: Science Advances
Article Title: Adaptable thermoresponsive polymer for long-term electrical coupling in plant electrophysiology monitoring
doi: 10.1126/sciadv.ady1400
Figure Lengend Snippet: ( A ) Procedure of drought stress and recovery experiment and photographs of plants under testing. Predrought is defined as 1 day before plants display wilting symptoms. ( B ) Amplitude of light-induced potential change for plants under 9 days of escalating drought (red) benchmarked against well-watered control plants (gray), showing an increase followed by decrease upon wilting. a.u., arbitrary units. ( C ) Light-induced potential change before and after inhibition of Ca 2+ channels in the same leaf. N = 3. ( D ) Light-induced potential change before and after inhibition of ROS production in the same leaf. N = 3. ( E ) Confocal images of intracellular Ca 2+ (top) and apoplastic ROS (bottom) during drought simulation, with quantification of the mean fluorescence intensity per region of interest (ROI) shown on the right. BF, bright field. Fluo-3 AM and 2′,7′-dichlorodihydrofluorescein diacetate (H 2 DCFDA): dyes used for visualizing Ca 2+ and ROS, respectively. Scale bar, 50 μm. CK, control plants; DR, drought-stressed plants. ( F ) Plant EP signals under the light and dark conditions with starting potential aligned for all samples on each day. N = 6. ( G ) Mean potential calculated from signals in (F) 1 hour after light on or light off, showing opposing trends with time under light and dark conditions. Dark mean potential changes before visual symptom onset on day 7. Solid lines in (C), (D), and (F) are mean values, and shadows represent SD. Box plots in (B), (E), and (G) display first quartile, median, and third quartile. Whiskers are drawn at 1.5 interquartile range distance. Means are denoted by empty squares. * P < 0.05; *** P < 0.001; **** P < 0.0001; n.s., not significant. Two sample t test.
Article Snippet: Intracellular Ca 2+ ions were detected using the fluorescent Ca 2+ indicator Fluo-3 AM (MedChemExpress, HY-D0716).
Techniques: Control, Inhibition, Fluorescence
